Journal: Cell and tissue banking

Article Title: Struggling with a cefazolin impregnation protocol of bone chips.

doi: 10.1007/s10561-025-10164-5

Figure Lengend Snippet: Fig. 1 An overview of the impregnation and elution protocol used. Winkler’s protocol (Winkler and Haiden 2017) is presented by the black arrows. The different time points of cefazolin impregnation are indicated by the grey dotted lines

Article Snippet: Materials Cefazolin sodium salt (purity 98%) and vancomycin hydrochloride (purity 98%) for UPLC-analysis were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques:

Journal: Cell and tissue banking

Article Title: Struggling with a cefazolin impregnation protocol of bone chips.

doi: 10.1007/s10561-025-10164-5

Figure Lengend Snippet: Fig. 2 Stability of vancomycin (a) and cefazolin (b) in newborn calf serum (75 mg/L) incubated at 4 °C, room temperature (RT) and 37 °C for 6 days. The results are expressed as a percentage of the reference

Article Snippet: Materials Cefazolin sodium salt (purity 98%) and vancomycin hydrochloride (purity 98%) for UPLC-analysis were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques: Incubation

Journal: Cell and Tissue Banking

Article Title: Struggling with a cefazolin impregnation protocol of bone chips

doi: 10.1007/s10561-025-10164-5

Figure Lengend Snippet: An overview of the impregnation and elution protocol used. Winkler’s protocol (Winkler and Haiden ) is presented by the black arrows. The different time points of cefazolin impregnation are indicated by the grey dotted lines

Article Snippet: Cefazolin sodium salt (purity 98%) and vancomycin hydrochloride (purity 98%) for UPLC-analysis were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques:

Journal: Cell and Tissue Banking

Article Title: Struggling with a cefazolin impregnation protocol of bone chips

doi: 10.1007/s10561-025-10164-5

Figure Lengend Snippet: Stability of vancomycin ( a ) and cefazolin ( b ) in newborn calf serum (75 mg/L) incubated at 4 °C, room temperature (RT) and 37 °C for 6 days. The results are expressed as a percentage of the reference

Article Snippet: Cefazolin sodium salt (purity 98%) and vancomycin hydrochloride (purity 98%) for UPLC-analysis were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques: Incubation

Journal: Cell and Tissue Banking

Article Title: Struggling with a cefazolin impregnation protocol of bone chips

doi: 10.1007/s10561-025-10164-5

Figure Lengend Snippet: a Influence of the cefazolin concentration of the impregnation solution (mg/mL) and impregnation time on the eluate cefazolin concentration after 24 h from securized fresh frozen bone. Bone chips were washed twice prior to elution. b Influence of the impregnation time and bone processing on the eluate concentration after 24 h using a cefazolin impregnation solution of 20 mg/mL (n = 2; results are presented as the mean ± SD). Bone chips were washed four times (SFFB and SLB) and five times (UFFB) prior to elution. c Elution of cefazolin as a function of time using a 20 mg/mL cefazolin impregnation solution for 4 h (n = 3; results are mean ± SD). Bone chips were washed four times after impregnation

Article Snippet: Cefazolin sodium salt (purity 98%) and vancomycin hydrochloride (purity 98%) for UPLC-analysis were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques: Concentration Assay

Journal: Cell and Tissue Banking

Article Title: Struggling with a cefazolin impregnation protocol of bone chips

doi: 10.1007/s10561-025-10164-5

Figure Lengend Snippet: Results of eluted cefazolin after 100 mg/mL solution impregnation. BC type A: bone chips impregnated after general CO 2 cleaning, BC type B: bone chips impregnated after freeze drying, BG type B: bone granules impregnated after freeze drying, BC type C: bone chips impregnated after gamma-irradiation. Each condition was performed in triplicate, and the results are given as the mean ± SD

Article Snippet: Cefazolin sodium salt (purity 98%) and vancomycin hydrochloride (purity 98%) for UPLC-analysis were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques: Irradiation

Journal: Cell and Tissue Banking

Article Title: Struggling with a cefazolin impregnation protocol of bone chips

doi: 10.1007/s10561-025-10164-5

Figure Lengend Snippet: Results of eluted cefazolin after 20 mg/mL solution impregnation. BC type A: bone chips impregnated after general CO 2 cleaning, BC type B: bone chips impregnated after freeze drying, BC type C: bone chips impregnated after gamma-irradiation, SFFB 4 h: securized fresh frozen bone chips impregnated in-house for 4 h. Each condition was performed in singlicate

Article Snippet: Cefazolin sodium salt (purity 98%) and vancomycin hydrochloride (purity 98%) for UPLC-analysis were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques: Irradiation

Journal: Cell and Tissue Banking

Article Title: Struggling with a cefazolin impregnation protocol of bone chips

doi: 10.1007/s10561-025-10164-5

Figure Lengend Snippet: Stability of cefazolin in newborn calf serum (NBCS) and aqueous solution (120 mg/L) incubated at 37 °C for 4 and 8 days respectively. The results are expressed as a percentage of the reference

Article Snippet: Cefazolin sodium salt (purity 98%) and vancomycin hydrochloride (purity 98%) for UPLC-analysis were purchased from Toronto Research Chemicals (Toronto, Canada).

Techniques: Incubation